Recognition Pattern of Thyroglobulin Autoantibody from Hypothyroid Dogs to Tryptic Peptides of Canine Thyroglobulin
نویسندگان
چکیده
منابع مشابه
Proliferative responses to canine thyroglobulin of peripheral blood mononuclear cells from hypothyroid dogs.
The immune responses of hypothyroid dogs to canine thyroglobulin (cTg) were evaluated for the proliferative ability of peripheral blood mononuclear cells (PBMC). PBMC from three hypothyroid dogs with high titers of thyroglobulin autoantibody (TgAA) and 3 clinically normal dogs were cultured with 5, 10, or 20 microg/ml of cTg for 72 hr. The proliferative responses of the cells were determined by...
متن کاملELISA for thyroglobulin in serum: recovery studies to evaluate autoantibody interference and reliability of thyroglobulin values.
An ELISA for measuring thyroglobulin (Tg) in serum was developed with polyclonal antibodies to Tg on the solid phase and two monoclonal antibodies to Tg as the second antibodies. The assay has a detection limit of 1 microgram/L, a within-run imprecision (CV) of <7%, and a between-run CV of < 10%. Parallelism of the assay was shown in dilution studies, in which the percent observed/expected valu...
متن کاملThe Properties of Thyroglobulin VIII. THE IODINATION OF THYROGLOBULIN
It has been shown that when thyroglobulin is iodinated, a marked intensification occurs in its absorption spectrum in the region of 300 to 3fXl rnp in alkaline solution (1). It is also well known that the absorption peaks of iodinated tyrosine and thyronine derivatives occur in this spectral zone (2). A procedure based on these factors has been developed which permits the simultaneous determina...
متن کاملThyroglobulin structure-function. Isolation and characterization of a thyroxine-containing polypeptide from bovine thyroglobulin.
Upon reduction and alkylation, 19 S bovine thyroglobulin gave rise to a family of polypeptides ranging in molecular weight from 10,000 to 330,000. T h i s group of polypeptides has been fractionated by chromatography on agarose gel in 6 M urea. After observing significantly increased iodine and thyroxine contents in the lowest molecular weight fraction obtained from the agarose gel-6 M urea chr...
متن کاملFree Thyroxine Concentrations by Equilibrium Dialysis and Chemiluminescent Immunoassays in 13 Hypothyroid Dogs Positive for Thyroglobulin Antibody
OBJECTIVE To determine if concentrations of free thyroxine (FT4) measured by semi-automated chemiluminescent immunoassay (CLIA) correspond to FT4 determined by equilibrium dialysis (ED) in hypothyroid dogs positive for thyroglobulin antibody (TGA). ANIMALS Thirteen TGA-positive dogs classified as hypothyroid based on subnormal FT4 concentrations by ED. METHODS Qualitative assessment of cani...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2003
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.65.1049